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Journal of Scientific Research and  Studies

Journal of Scientific Research and Studies Vol. 4(12), pp. 366-372, December, 2017

ISSN 2375-8791

Copyright © 2017

Author(s) retain the copyright of this article

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Full Length Research Paper

Molecular study of the population structure and geographic distribution of Bemisia tabaci (Hemiptera: Aleyrodidae) in the North East Arid Zone of Nigeria

 

Gadzama U. N.1*, Anaso C. E.1, Turaki J. M.1 and Zakariya D.2

1Department of Biological Sciences, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria.
2Department of Crop Protection, Faculty of Agriculture, University of Maiduguri, Maiduguri, Nigeria.
 

*Corresponding author. E-mail: ugadzama@yahoo.com
 

Accepted 18 December, 2017

 

 Abstract

 

This study was conducted to determine the population structure and geographic distribution of Bemisia tabaci (Whitefly) in north-east Nigeria, West Africa. Two localities each were selected from three States of North-eastern Nigeria for sample collections and determination of population structure and distribution. The localities include Toro and Wuro wasse in Bauchi State, College of Agric farm center (COAG) and Tudun wada in Borno State and Dadin kowa and Kwadon in Gombe State. Population genetic variability was performed on a total of 240 whiteflies using Polymerase Chain Reaction (PCR) and microsatellite DNA analysis on mtCOI as target genes using BEM12, BT-e49 and BT-b103 primers. The results of genetic characterization based on PCR and mtCOI nucleotide sequence identity revealed a clear geographic distribution of three biotypes B, Q and unknown. Comparison of amplified DNA size (bp) and mitochondrial cytochrome oxidase I subunit (mtCOI) sequence revealed a similarity of 97.2– 99.5% of B and Q biotypes found in this study with others elsewhere while the unknown biotype probably heterozygote between Q and B were few in all locations. The average number of alleles (NA) per locus was estimated to be 1.66. The average NA ranged from 1.672 alleles (Toro) to a maximum of 2.116 al­leles (Dadin kowa). The observed heterozygosity (HO) varied among populations with a mean of 0.832. The inter-population variation index (FST) = 0.34.

Key words:
Bemisia tabaci, biotypes, distribution, microsatellite markers, mitochondrial, Cytochrome oxidase I subunit.

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