Scientific Research and Studies Vol. 4(10), pp. 264-268,
Copyright © 2017
Author(s) retain the
copyright of this article
Full Length Research Paper
Study on the
antibacterial properties of probiotic bacteria isolated from
human breast milk
Okoro C.I.1*, Ihenetu F.C.2, Ogwunga C.C.3 and
Microbiology/Parasitology, Federal Medical Center Owerri, Nigeria.
2Department of Microbiology, Federal University of Technology, Owerri,
3Department of Biotechnology, Federal University of Technology,
4Department of Medical Laboratory Science, Madonna University, Elele,
*Corresponding author. E-mail:
Accepted 21 September, 2017
Human breast milk is said to be rich in various bioactive
compounds which promote the maturation of immune system as well
as developing body’s defense against infections in infants.
Breast milk was obtained from six different volunteer
breastfeeding mothers, grouped into sample [A, B, C, D, E and
F]. Result showed that sample D had the highest LAB count of 1.6
x 108 cfu/ml (P>0.05) while sample A had the least
LAB count of 1.3 x 106 cfu/ml (P>0.05). The isolates
from the breast milk samples were characterized as
Lactobacillus plantarum and Lactobacillu pentosus and
were observed as potential probiotics. These bacteria were
facultative anaerobic, gram positive, catalase negative and non-endospore
forming. They showed tolerance against 0.3% bile concentration
and 1-10% NaCl. Sugar fermentation patterns of the isolated
bacteria also greatly varied. The LAB isolates; L.
plantarum and L. spentosus showed a great
antimicrobial effect on all the indicator organisms.
Klebsiella sp. had the highest (P<0.05) susceptibility of
16±1.41 mm and 14±8.48 mm with L. plantarum and L.
pentosus, respectively while Escherichia coli had a
greater resistance (P<0.05) of 11±0.7 mm and 6 ±2.82 mm with
L. plantarum and L. pentosus, respectively. The
addition of breast milk probiotics to infant formulas could be a
new alternative to mimic some of the functional effects of human
milk in children who are not breastfed.
Breast milk, lactic acid bacteria, API50CHL, antimicrobial,
Lactobacillus plantarum, Lactobacillus pentosus.
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