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Journal of Scientific Research and  Studies

Journal of Scientific Research and Studies Vol. 4(10), pp. 264-268, October, 2017

ISSN 2375-8791

Copyright © 2017

Author(s) retain the copyright of this article


Full Length Research Paper

Study on the antibacterial properties of probiotic bacteria isolated from human breast milk


Okoro C.I.1*, Ihenetu F.C.2, Ogwunga C.C.3 and Dunga K.E.4

1Department of Microbiology/Parasitology, Federal Medical Center Owerri, Nigeria.
2Department of Microbiology, Federal University of Technology, Owerri, Nigeria.
3Department of Biotechnology, Federal University of Technology, Owerri, Nigeria.
4Department of Medical Laboratory Science, Madonna University, Elele, Nigeria.

*Corresponding author. E-mail:

Accepted 21 September, 2017




Human breast milk is said to be rich in various bioactive compounds which promote the maturation of immune system as well as developing body’s defense against infections in infants. Breast milk was obtained from six different volunteer breastfeeding mothers, grouped into sample [A, B, C, D, E and F]. Result showed that sample D had the highest LAB count of 1.6 x 108 cfu/ml (P>0.05) while sample A had the least LAB count of 1.3 x 106 cfu/ml (P>0.05). The isolates from the breast milk samples were characterized as Lactobacillus plantarum and Lactobacillu pentosus and were observed as potential probiotics. These bacteria were facultative anaerobic, gram positive, catalase negative and non-endospore forming. They showed tolerance against 0.3% bile concentration and 1-10% NaCl. Sugar fermentation patterns of the isolated bacteria also greatly varied. The LAB isolates; L. plantarum and L. spentosus showed a great antimicrobial effect on all the indicator organisms. Klebsiella sp. had the highest (P<0.05) susceptibility of 16±1.41 mm and 14±8.48 mm with L. plantarum and L. pentosus, respectively while Escherichia coli had a greater resistance (P<0.05) of 11±0.7 mm and 6 ±2.82 mm with L. plantarum and L. pentosus, respectively. The addition of breast milk probiotics to infant formulas could be a new alternative to mimic some of the functional effects of human milk in children who are not breastfed.

Key words:
Breast milk, lactic acid bacteria, API50CHL, antimicrobial, Lactobacillus plantarum, Lactobacillus pentosus.

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Vol. 4 Issue 10

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