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Journal of Scientific Research and  Studies

Journal of Scientific Research and Studies Vol. 5(1), pp. 21-25, January, 2018

ISSN 2375-8791

Copyright 2018

Author(s) retain the copyright of this article


Full Length Research Paper

Evaluation of antimicrobial synergy of scent plant (Ocimum gratissimum) and guava (Psidium guajava) extracts against some intestinal pathogens


Ademokoya A. A.* and Mekoma A. A.

Department of Microbiology, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria.

*Corresponding author. E-mail:

Accepted 27 December, 2017




Antimicrobial drugs resistance by pathogenic microorganisms, prevalent in orthodox medicine in recent time, has brought about search for natural alternative phytochemical; otherwise called medicinal plants, for the treatment of diseases and infections caused by these microbes. Therefore, this study was designed to evaluate the efficacy of scent plant (Ocimum gratissimum) and guava (Psidium guajava) extracts synergistically against intestinal pathogens; Escherichia coli O157:H7, Salmonella typhi, Pseudomonad aureginosa and Staphylococcus aureus. Moreover, the sensitivity of the pathogens to different concentrations of the plants extracts was carried out using agar diffusion technique, and the zone of inhibition was measured in millimeter. Concerning the study data, One-way ANOVA was done using statistical software package SPSS version 20. The percentage inhibition synergistically mediated by O. gratissimum and P. guajava against the pathogens is arranged in ascending order as follows; 5% ethanolic extraction inhibited 114%  E. coli O15:H7, 140% S. typhi, 152% P. aureginosa, and 100% S. aureus. For 5% methanol extraction, 100% E. coli O157:H7 was inhibited, followed by 133% S. typhi, 140% P. aureginosa, while S. aureus had the lowest inhibition of 127%. Moreover, the aqueous extraction of the plants extract had the following inhibition; S. aureus 50%, E. coli O157:H7 43, P. aureginosa 40% while the lowest inhibition was found in S. typhi 33%. In conclusion, this study has been able to show that the synergy of O. gratissimum and Psidium guajava mediated excellent inhibitory capacity against the enteric pathogens assayed in this study. Therefore, these plants extracts could be adopted for the control of infections caused by these pathogens.

Key words: Ocimum gratissimum, Psidium guajava, extracts, synergy, enteric pathogens.

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