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Journal of Scientific Research and  Studies

Journal of Scientific Research and Studies Vol. 5(3), pp. 58-64, March, 2018

ISSN 2375-8791

Copyright 2018

Author(s) retain the copyright of this article

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Full Length Research Paper

Population structure of the brown planthopper, Nilaparvata lugens (Homoptera: Delphacidae) based on PCR analysis in West Sumatra, Indonesia

 

Enie Tauruslina A1*, Trizelia2, Yaherwandi2 and Hasmiandy Hamid2

1Department of Agriculture, Food and Horticulture Plant Protection Office, West Sumatera, Indonesia.
2Department of Plant Protection, Faculty of Agriculture, University of Andalas, Padang, West Sumatra, Indonesia.
 

*Corresponding author. E-mail: etauruslina@yahoo.com

Accepted 14 March, 2018

 

 Abstract

 

This research was conducted to determine the population structure of brown planthopper (Nilaparvata lugens) in the Province of West Sumatra, Indonesia. N. lugens sample location was selected from fourteen districts for determining the sample collection of the population structure. The localities were Tanjung Mutiara, IV Nagari, Lubuk Basung, Kinali, Lengayang, Koto VII, X Koto Singkarak, Rambatan, Sitiung, Koto Baru, Nanggalo, Kuranji, Talawi and Sungai Geringging. Population genetic variability was performed using Polymerase Chain Reaction (PCR) and DNA analysis using SSRNLS24, SSRBM1262 and SSRBPH06 primers. The results of genetic characterization based on PCR revealed a clear population structure. Population structure of N. lugens is diversed based on PCR SSR analysis. Amplification of N. lugens gene shows that sample 1 to 14 of DNA bands was a consistent pattern of DNA bands. The dendrogram population of N. lugens was eight samples, with genetic similarity at > 0.50.

Key words:
Rice, marka SSR, phylogenetic, Nilaparvata lugens.

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Vol. 5 Issue 3
 

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