Scientific Research and Studies Vol. 5(3), pp. 65-72,
Copyright © 2018
Author(s) retain the
copyright of this article
Full Length Research Paper
prevents inflammatory effects from carrageenan-induced knee
joint synovitis in rats through A1 adenosine
receptor, as well as lipid and protein oxidative damages
Gabriel Takon Oru1,
Renate Viebhan-Haensler2, Yanet Hernández Matos1, Daylin
Díaz Rodríguez3, Mayté Casanova Orta1 and Olga Sonia León
1Pharmacy and Food
Institute, University of Havana, Havana 10 400, Cuba.
2Medical Society for the Use of Ozone in Prevention and Therapy,
Iffezheim/Baden-Baden D-76473, Germany.
3Center for Control of Drug and Medical Devices (CECMED), Ministry of
Public Health, Havana 10 300, Cuba.
*Corresponding author. E-mail:
Tel: (537) 7 272 77 26
Accepted 22 March, 2018
Medical ozone reduced inflammation, IL-1β, TNF-α mRNA levels and
oxidative stress in experimental arthritis. The aim of this
study was to investigate whether medical ozone could reduce
inflammation through A1 adenosine receptor, as well
as by decreasing oxidative stress in carrageenan-induced knee
joint synovitis. Wistar rats were used for the study. Synovitis
was induced by 50 µl intra-articular injection of 3% carrageenan.
The joint inflammation, the histopathological characterization
of knee joint and redox markers in supernatants of spleen
homogenates were determined. In order to evaluate the role of A1
adenosine receptor, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX)
was tested. The results show that medical ozone prevented
inflammation, reduced cell infiltration, and protected against
oxidative protein damage and lipid peroxidation. DPCPX
counteracted the protective effects of ozone. In conclusion,
medical ozone protected against the inflammatory response in
experimental synovitis. The protective effects of ozone involved
A1 adenosine receptor activity and the control of
oxidative injury to biomolecules.
Key words: Ozone, inflammation, adenosine, oxidative
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